Composite

Part:BBa_K2683028

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-10)


MyoKinase (MK) in pSB1C3

Myokinase (MK) is a enzyme that transfers phosphate goups in order to convert adenine nucleotides (ATP, ADP, and AMP). Due to its function, this protein is essential for maintaining cellular homeostasis and energy levels [1]. This part is codon optimized for use in E.coli , has a N-terminal His tag and has a T7 promoter, medium strength RBS and double terminator. This part was originally designed by the 2017 Lethbridge iGEM team ( http://2017.igem.org/Team:Lethbridge). The part was initially characterized in the pUC57 plasmid (https://parts.igem.org/Part:BBa_K2443033).

This part was improved by placing it into the pSB1C3 plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 749
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None